Hydrogen Bond Chromatography – A New Tool for Enhanced Separation of Proteins from Virus Particles and Other Very Large Biologics

CIMmultus ADC monoliths from BIA Separations are the first chromatography products in the field to exploit hydrogen bonding as the primary binding mechanism. These products bring a unique new selectivity to all purification challenges, but they are especially distinctive in their ability to retain large biomolecules more strongly than small ones. They enhance removal of fragments, aggregates, and viruses from proteins, and they enhance removal of proteins and other small contaminants from viruses and other very large biologics. H-Bond ADC also supports outstanding DNA removal.

Hydrogen bonds are polar, and they can be dissociated with salts, but they require higher concentrations than ion exchangers. This makes H-Bond ADC more tolerant of salt in applied samples. Most samples can be applied at pH 7.0 in 100 mM NaCl. Large biologics often can be applied in 200–300 mM NaCl. Salt tolerance increases at lower pH values. This simplifies sample preparation and minimizes the need to dilute or buffer-exchange samples between chromatography steps.

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