Failure to detect breaches in chromatography column performance can be disastrous during large-scale commercial manufacturing. Our company uses algorithm modeling for near–real-time monitoring of column packing quality and sensitive detection of column-integrity breaches. The approach enables us to mitigate risks early on, save cost and time, and thereby deliver consistent product quality and purity during manufacturing. Here we discuss three case studies in which predictive algorithm modeling using moment analysis and direct transition analysis (DTA) helped us monitor column integrity…