Author Archives: BPI Contributor

Using Slope Spectroscopy for Faster, Repeatable, More Accurate Protein Concentration Measurements

This webcast features: Joe Ferraiolo, Bioanalytics Applications Manager, Repligen Traditional capture chromatography titer measurements that involve dilution requirements represent the highest single source of error in traditional UV analysis. Slope spectroscopy requires no sample preparation or dilution, saving substantial time without changing any other aspects of the assay. This webinar will demonstrate how variable pathlength slope spectroscopy using the SoloVPE™ device can significantly reduce sample prep and process time, while maintaining high repeatability and accuracy. Just fill out the form…

Highly Sensitive Host Cell Protein Analysis Using µPAC™ LC-MS

This webcast features: Dr. Koen Sandra, Scientific Director, Research Institute for Chromatography Within a rapidly growing biopharmaceutical market, there is an increasing demand for the development of a rising number of biotherapeutic protein drugs, such as monoclonal antibodies (MAbs). During the development and production of biotherapeutic proteins, typically using mammalian cell lines, such as Chinese hamster ovary (CHO) cells, additional proteins are expressed as well by the host cell. These so-called host cell proteins (HCPs) will need to be removed,…

Cell Culture Media Analysis Using a Handheld Raman Analyzer with Onboard Chemometrics

This webcast features: Orville Dean Stuart, Technical Regional Sales Manager, Thermo Fisher Scientific This webinar presents the use of a handheld Raman analyzer with onboard chemometrics to verify the identify and monitor the quality and batch-to-batch variability of both dry powder chemically defined media and ready-to use liquid cell culture media. While scans of cell culture media using Thermo Scientific’s TruScan RM handheld Raman analyzer produce spectra with good Raman features, due to similar constituents and formulations, the TruTools add-on…

Investigation of Hitchhiking Impurities Removed by the 3M EMPHAZE AEX Hybrid Purifier

This webcast is hosted by: Dr. Hani El-Sabbahy, Biopharmaceutical Application Engineering Specialist, Separation and Purification Sciences Division, 3M United Kingdom PLC 3M™ Emphaze™ AEX Hybrid Purifier is an all-synthetic clarifier consisting of a high capacity Q-functional hydrogel and a 0.2 μm polyamide membrane. The open structure and high charge capacity can handle turbid feeds, resulting in soluble and insoluble contaminant removal. Using data generated by sophisticated LC-MS/MS techniques, this webinar will explore the impact of deploying the Emphaze AEX Hybrid…

Packing Rigid POROS Chromatography Resins: Efficient and Fast Method to Get Optimal Results

This webcast features: Dr. Anna Le Bris, Senior Application Specialist, Bioproduction, Thermo Fisher Scientific POROS resins are widely used in commercial bioprocesses for the purification of large molecules, in both the capture and polish steps of downstream processing. POROS chromatography resins are based on a rigid polymer, allowing them to withstand very high pressure and be mechanically and chemically stable. Because POROS is a rigid bead, it can be packed in cGMP columns with volumes up to 1000 L. POROS…

Robust Performance of OPUS® Columns Pre-Packed with CHT™ Resin

This webcast features: Fletcher Malcom, Product Line Leader – Chromatography, Repligen Ceramic hydroxyapatite (CHT™) resins are used in processes where unique selectivity is desired for purification of mAbs or recombinant proteins. Until recently, shipping columns pre-packed with CHT™ resin provided challenges due to the fragile nature of the chromatographic bed. To overcome these challenges, Repligen has developed a proprietary packing method for CHT™ resins which maintains chromatographic performance during transit. In this presentation, packing performance and shipping validation data will…

Scalable Purification Strategies for Viral Vectors and Vaccines

This webcast features: Mark A. Snyder, Ph.D., R&D Manager, Process Chromatography Applications, Bio-Rad Laboratories Large-scale downstream processing of viruses can face a range of obstacles different from those of many biotherapeutics. These challenges mostly arise from the size and complexity of the virus, which can affect product purity and recovery. Improvements of purification tools are necessary to overcome these challenges and must be engineered for easy scalability to meet manufacturing demands. Dr. Mark Snyder will discuss the difficulties when developing…

The Impact of Rapid Virus Quantification in the Development and Manufacture of Advanced Therapies

This webcast features: Antje Schickert, Product Manager, Virus Analytics, Sartorius Stedim Biotech Viral vectors, such as lenti- and adeno-associated viruses, are increasingly being used for the production of cell and gene therapies.  In-depth sample characterization and precise enumeration of total viral vectors is needed throughout the manufacturing process and particularly for the final formulation.  Learn how rapid accurate enumeration of the total virus provides timely information on titer leading to accelerated process development and increased efficiency of viral vector manufacturing.…

Analytical Power Tools Open Upstream Bioprocessing Bottlenecks

The blockbuster success of antibody-based therapeutics for autoimmune diseases, inflammatory diseases and immuno-oncology accelerated the high stakes monoclonal antibody (mAb) development race. Clone selection is a significant upstream bottle-neck slowing bench-to-bedside development progress for new mAb-based therapeutics. Cy-Clone™ PLUS, a validated assay kit accelerates human mAb production cell-line generation, significantly reducing the time to industrial-scale productivity. Cy-Clone PLUS assay combined with the Intellicyt® iQue platform and integrated ForeCyt® Software are powerful analytical tools that inform rapid go/no-go decisions with multiparameter,…

Ask the Expert: A New AEX Mixed-Mode Chromatography Resin for High Selectivity and Recovery

In an Ask-the-Expert webinar on 16 May 2019, Xuemei He (R&D manager of chromatography media chemistry at Bio-Rad Laboratories, Inc.) introduced the Nuvia aPrime 4A mixed-mode chromatography resin. Its macroporous particles exhibit good pressure-flow properties in addition to optimal ligand density and hydrophobicity. So this versatile resin offers effective separation of target from impurities in both flow-through and bind–elute modes, with good recovery of target proteins under gentle purification condition. He’s Presentation The resin is a hydrophobic anion exchanger with positively…