July 2009

Comparability Between the Mobius® CellReady 3-L Bioreactor and 3-L Glass Bioreactors

Figure 1: The Mobius CellReady 3-L bioreactor is a single-use, stirred-tank bioreactor ideal for bench-scale cell culture. The CellReady 3-L bioreactor is preassembled and gamma irradiated, significantly reducing turnaround times typically associated with glass bioreactors. There is no need to clean, assemble, or autoclave between experiments. The CellReady 3-L bioreactor ensures maximum operational flexibility with prefitted, weldable tubing and vent filter; two sparging options; and compatibility with most standard bioreactor controller configurations. Designed to replace traditional glass, bench-scale bioreactors, the…

Improving Peptide Mapping

Figure 1: Peptide mapping is the workhorse technique in biopharmaceutical analysis, offering the comprehensive characterization of biopharmaceutical products. Its applications include the identification of proteins based on the elution pattern of peptide fragments, the determination of posttranslational modifications, the confirmation of genetic stability, and the analysis of protein sequences when interfaced to a mass spectrometer. In a typical peptide mapping workflow, a protein is first digested with trypsin or other protease to generate peptides, which are separated by liquid chromatography,…