September 2011

Key Aspects of Enzyme Activity and Steady-State Kinetics

Living systems rely on enzymes to perform many essential functions for survival. One prime example is digestion, the conversion of food into energy. Each enzyme possesses specific requirements for the types of molecules that it can use as substrates or reactants to convert to products. Here, I provide some basic information about enzymes, explain their biochemical parameters (e.g., kinetic parameters) and significance for characterization, and review related assays currently available to the bioprocess industry. Lactose intolerance is a common enzyme…