Laboratory Equipment

Evaluating AMMP Protein A Assays: Comparing Sample Preparation Methods, Ligand Performance & Assay Performance

Regulations governing the production of biopharmaceuticals require high levels of purity for processes utilizing Protein A affinity chromatography. It has been shown that Protein A in the presence of IgG forms a PA/IgG complex that interferes in the traditional immunoassay format for detecting Protein A. In this work we evaluated the AMMP Protein A Assay using two popular sample preparation methods to dissociate the ProteinA/IgG complex. The AMMP assay was also tested with Protein A ligands from multiple sources including…

Optical Oxygen and pH Sensors for Monitoring Biofermentation Processes

Advances in high-performance sensor materials and optoelectronics have enabled optical sensors for use in markets including the life sciences, environmental, food and beverage, process control and biotechnology. Compared with traditional electrochemical sensing techniques such as galvanic, paramagnetic and fuel cell sensors, these optical sensors can be made in small and customizable form factors such as probes and self-adhesive patches. The sensors also have fast response, provide long-term stability and are chemically inert. This experiment demonstrates the viability of optical sensors…