Improving IEX Throughput and Performance with Differentiated Chromatography Sorbents

As upstream processing becomes more optimized, it increases the amount of impurity and contaminants to be removed from large volumes of feedstock. With this added pressure on downstream processing, there is a pressing need for more efficient chromatography operations.

In this paper, the authors describe how they characterized differentiated selectivity of Q and S HyperCel ion-exchange sorbents. They evaluated the dynamic binding capacity in various pH, conductivity, and residence time conditions; carried out a separation of a model mixture to further characterize the sorbents’ specific selectivity; and report on a real case application in which one was used in a capture step for purification of a recombinant protein from an E. coli lysate.

Improving Downstream Processing Using Membrane Chromatography

The chromatographer is faced with a variety of anion and cation exchange phases that include a limited range of functional groups. These groups are attached to a wider range of base matrices, such as sorbents for column applications, monoliths and membranes. When ion-exchange membranes are used in chromatography devices, they demonstrate very high usable flow rates with enhanced throughput. Additionally the pre-packed format of these columns enables ease of use and unit-to-unit consistency. It also allows for a disposable chromatographic processes that eliminates costly and time-consuming cleaning validation.

During this on-demand webcast, diverse case studies will be presented that feature applications ranging from contaminant clearance to capture of target molecules in downstream purification processes. These studies highlight scalability, fast and efficient capture of large molecules, and improved process economics. When used in these applications, ion-exchange membranes proved a demonstrable alternative to packed bed chromatography and is better suited for subsequent purification processes..

Join Ajay R. Lajmi of Pall Life Sciences as he takes an in-depth look at the trends and challenges facing downstream processing and illustrates the benefits of membrane chromatography vs. resin chromatography.