BPI White Papers

Delivering Fit-for-Purpose Biomanufacturing CHO Cell Lines

A prerequisite for the successful manufacture of a biopharmaceutical in mammalian cells is a cell line development strategy that selects a cell line with the most desirable properties from a heterogeneous transfectant population. Desirable attributes include the stable, high expression of product with the appropriate critical product quality attributes rapidly, reproducibly and with relative ease. Obtaining a host cell line that inherently exhibits such desirable biomanufacturing attributes can therefore have a significantly positive effect on the identification of recombinant cell…

Assessing Immunogenicity Using Ex Vivo T cell Assays

This article discusses Antitope’s developed highly sensitive ex vivo T cell assay technology that can detect T cell responses against T cell epitopes in therapeutic proteins. These assays can be used to select non-immunogenic lead proteins as well as support the optimisation of leads by engineering out T cell epitopes

CaPure-HA Resin for Aggregate Removal from Monoclonal Antibodies

Scientists in the field of process chromatography methods development are constantly on the lookout for better and more selective ways to remove aggregates and other process related impurities from monoclonal antibody monomer. The data presented in the following paper demonstrates the capabilities of CaPure-HA to remove degradation products, dimer, and higher order aggregates from the monomer of a protein A purified IgG1 monoclonal antibody.

Multitron Pro: The Professional Incubation Shaker for Any Application

The Multitron Pro and the Multitron Cell manage to combine flexibility, user-friendliness and operational reliability with  optimum space utilization. A number of innovative options for cooling, precise CO2 control, illumination and hygienic direct steam humidification guarantee the ideal conditions for cultures. With around 500 different designs we meet almost every customer requirement. We are also more than happy to provide special configurations. INFORS HT provides you with Swiss quality with a modern design and a high technological level and environmentally friendly energy…

Scalable Protein Production Using Flow Electroporation

Many transient gene expression (TGE) methods produce insufficient protein quantities for full use within biotherapeutic and vaccine development pipelines. MaxCyte’s proprietary flow electroporation provides a universal means of highly efficient TGE for the rapid production of large-scale quantities of proteins, antibodies, antibody-like molecules, virus-like particles (VLPs), and vaccines. In addition, the platform can be used for generation of stable pools and stable cell lines that can greatly streamline biotherapeutic and vaccine development. Flow electroporation (co)transfects a wide range of cells,…

Efficacy and Utility of New EXP-Pak Closed-System Disposable Cell Expansion Bags Designed for Cell Therapy Applications

Clinical cellular therapy applications often times require a cell expansion or maturation step prior to use. Traditionally, cell expansion or cell culture is performed in “open” systems including multi-well culture dishes or tissue culture flasks. These “open” steps present risks and are not ideal for larger-scale manufacturing. The new EXP-Pak™ cell expansion container offered by Charter Medical, Ltd. is a closed-system, gas permeable bag intended for expansion and culture of non-adherent cells. The data demonstrate that the EXP-Pak™ cell expansion…