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How Molded Manifold Assemblies Prevent Leaks & Costly Consequences

Molded tubing manifolds have roles to perform over and above being a transport vehicle for liquids. The assemblies must maintain leak-proof flow. They need to meet sterility requirements and help limit the need for cleaning and associated validations. They also must be cost effective, simple to use and efficient. When compared with hand-assembled tubing sets, the advantages of molded tubing manifold assemblies are clear. Tubing sets made from multiple components present a risk of leaks at every juncture. With barbed…

Development of a Novel Cold-Chain Tubing, FP-FLEX™, and Single-Use Freezing Bag: For Working Cell Banks Enabling Closed-System Processing to Temperatures As Low As –196 °C

Working cell banks (WCBs) are commonly applied to initiate cell culture manufacturing campaigns to produce therapeutic proteins. Those campaigns typically begin with the inoculation of cells previously cryopreserved in vials. Although vials are typically used to establish WCBs and initiate manufacturing campaigns, they are not optimal for the growing demands of commercial production.

New Device for Biomass Monitoring in Shake Flask Culture

We developed a novel system for online biomass measurement in shake flasks based on near 360° scattered light detection. The optosensoric components were combined with the established PreSens SFR Shake Flask Reader technology, so biomass, pO2 and pH can be monitored non-invasively through the flask bottom. First application of the prototype SFR vario on Escherichia coli K12 and Kluyveromyces marxianus culture resulted in highly reproducible online biomass data, with relative errors matching those of standard offline OD measurements.

Three Steps to Start You on the Path to Obtaining the Best Recombinant CHO Cell Line

When developing efficient, high quality biomanufacturing processes, it is imperative that the expression system (‘host cell line’ and ‘vector’) and the process to isolate and identify the best clonal cell line (‘clonal cell line development process’) are of the highest quality. At FUJIFILM Diosynth Biotechnologies a team of scientists has invested more than 20 FTE years undertaking innovation work which has culminated in the development of a new host cell line and vector, along with an optimised clonal cell line…

Flow Electroporation Capabilities and Case Studies: Rapid GPCR Screening and Functional Ion Channel Assays

MaxCyte’s proprietary flow electroporation technology has been successfully applied in ex vivo cell therapy (1) and drug discovery pipelines where reproducibility, efficiency and the need for increased cell numbers are critical. This technical paper discusses the merits of transient transfection using flow electroporation versus other recombinant expression approaches and its application in hit identification and lead optimization programs. Data are presented demonstrating the key features of the MaxCyte® STX™ Scalable Transfection System, including broad applicability, scalability, and the production of…

Delivering Fit-for-Purpose Biomanufacturing CHO Cell Lines

A prerequisite for the successful manufacture of a biopharmaceutical in mammalian cells is a cell line development strategy that selects a cell line with the most desirable properties from a heterogeneous transfectant population. Desirable attributes include the stable, high expression of product with the appropriate critical product quality attributes rapidly, reproducibly and with relative ease. Obtaining a host cell line that inherently exhibits such desirable biomanufacturing attributes can therefore have a significantly positive effect on the identification of recombinant cell…

Assessing Immunogenicity Using Ex Vivo T cell Assays

This article discusses Antitope’s developed highly sensitive ex vivo T cell assay technology that can detect T cell responses against T cell epitopes in therapeutic proteins. These assays can be used to select non-immunogenic lead proteins as well as support the optimisation of leads by engineering out T cell epitopes

Multitron Pro: The Professional Incubation Shaker for Any Application

The Multitron Pro and the Multitron Cell manage to combine flexibility, user-friendliness and operational reliability with  optimum space utilization. A number of innovative options for cooling, precise CO2 control, illumination and hygienic direct steam humidification guarantee the ideal conditions for cultures. With around 500 different designs we meet almost every customer requirement. We are also more than happy to provide special configurations. INFORS HT provides you with Swiss quality with a modern design and a high technological level and environmentally friendly energy…

Scalable Protein Production Using Flow Electroporation

Many transient gene expression (TGE) methods produce insufficient protein quantities for full use within biotherapeutic and vaccine development pipelines. MaxCyte’s proprietary flow electroporation provides a universal means of highly efficient TGE for the rapid production of large-scale quantities of proteins, antibodies, antibody-like molecules, virus-like particles (VLPs), and vaccines. In addition, the platform can be used for generation of stable pools and stable cell lines that can greatly streamline biotherapeutic and vaccine development. Flow electroporation (co)transfects a wide range of cells,…