Ceramic hydroxyapatite (CHT™) is used for protein purification owing to its superior removal of all process-stream impurities (aggregates, HCP, viruses, endotoxin, protein A, DNA, etc.). At commercial scale CHT performance can be impacted due to the release of protons during elution. This proton release lowers mobile phase pH which may impact the robustness of CHT particles. We have developed a novel, patent-pending technology called the surface neutralization system (SNS), which desorbs protons from the surface of CHT prior to elution. We have demonstrated that the SNS technique extends the useful life of CHT and prevents pH drop during elution without desorbing the target protein when the target protein is a monoclonal antibody or polyclonal human IgG. This poster presents data demonstrating that this technology does not impact the common quality outputs typically monitored during antibody purification.
For questions about this poster, please contact:
Mark A. Snyder, Manager
Process R&D Applications Group