Figure 1:
Overcoming the bottleneck is a key statement often heard in the context of bioprocessing. Acceleration of both up- and downstream processes have therefore become a general need in the biopharmaceutical industry. Not only because this will release resources to comply with the increasing numbers of drug candidates, but also because this will shorten the time-to-market, giving more time to realize a return on investment before the patent expires.
Thus there is a need to apply new innovative methods to accelerate process development. This article describes how automation in the different areas of process development can contribute to achieving these targets and presents an overview of the applications currently in use on Tecan Freedom EVO® robotic workstations:
(*
Application is not described in this article. For more information, please refer to the Tecan contact at the end of this article
.)
Upstream Process Development
A generic framework for rapid identification and optimization of factors affec...
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Peptide mapping is the workhorse technique in biopharmaceutical analysis, offering the comprehensive characterization of biopharmaceutical products. Its applications include the identification of proteins based on the elution pattern of peptide fragments, the determination of posttranslational modifications, the confirmation of genetic stability, and the analysis of protein sequences when interfaced to a mass spectrometer.
In a typical peptide mapping workflow, a protein is first digested with trypsin or other protease to generate peptides, which are separated by liquid chromatography, most commonly reversed-phase LC, and then detected by either UV/VIS or MS. Compared with a traditional LC-UV/VIS approach, LC/MS analysis significantly enhances the information content available from a peptide mapping experiment. Measurement by MS can differentiate coeluting peptides under a chromatographic peak, and can identify and locate low-abundance modifications and single amino acid variants of a protein by...
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