Voices of Biotech
Podcast: MilliporeSigma says education vital to creating unbreakable chain for sustainability
MilliporeSigma discusses the importance of people, education, and the benefits of embracing discomfort to bolster sustainability efforts.
June 10, 2020
Date: Jun 10, 2020
Duration: 20 Min
Sponsored by Repligen
This webcast features: Michael Bransby, R&D Director of Process Technology, Repligen
In a joint collaboration, Oxford Biomedica and Repligen increased the yield of viral vectors from suspension-cultured bioreactors several fold using tangential-flow depth filtration (TFDF). The yield for a single clarification step was 95% as compared to 70% by standard depth filtration. The TFDF tubular format and low shear enabled further yield increases through multiple harvests from the same seeding. A single seeded bioreactor produced two harvests of 95% and 85%. The 180% yield represents a 2.5-fold increase over a single depth-filtration harvest at 70%. The process was shown to scale from 5 L to 50 L with an average flux of 750 LMH and three different lentiviral model systems, demonstrating broad applicability. Logistics and execution required a single filter, three AseptiQuik connections, and typically 15–20 minutes for setup and breakdown, resulting in a four-fold reduction in required footprint as compared to conventional depth filtration.
Watch the recorded webcast now.
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