Voices of Biotech
Podcast: MilliporeSigma says education vital to creating unbreakable chain for sustainability
MilliporeSigma discusses the importance of people, education, and the benefits of embracing discomfort to bolster sustainability efforts.
- Sponsored Content
- Manufacturing
BPI Contributor
February 18, 2021
20 Min View
Date: Feb 18, 2021
Duration: 20 Min
Sponsored by Sartorius
This webcast features: Aleš Štrancar, Managing Director, BIA Separations, a Sartorius Company
In vitro transcription (IVT), the enzymatic process used for the production of mRNA vaccines, differentiates itself from biological fermentation processes by the need for linearized plasmid DNA. The linear isoform is produced with restriction enzymes from open-circular and supercoiled plasmid DNA. Employing a traditional pDNA manufacturing process, which removes linear and open-circular isoforms, will reduce production yield. However, when plasmid DNA and mRNA are treated as a single production process, purification steps can be optimized, yields improved, and overall production costs reduced.
Three key takeaways:
Learn about a new purification approach starting from E. coli through to mRNA production, resulting in improved recoveries, extra-low protein impurities, and very efficient dsRNA removal.
Get insights into how pDNA linearization based on monoliths is leveraged prior to and during polishing in this purification strategy.
Understand the use of in-process analytics and mRNA purification tools for improved contaminant removal.
Just fill out the form below to watch the recorded webcast now.
About the Author(s)
You May Also Like
Upcoming Webcasts
Jun 17 - Jun 20, 2024
Jun 17 - Jun 20, 2024
Our Biopharmaceutical School is the perfect course to gain a well-rounded understanding of the Biopharmaceutical industry. Over three days, you will understand the principle steps in the biopharmaceutical lifecycle, including: raw material selection, up and downstream processes and core technology. Additionally, you will be familiarised with the latest industry trends, including Industry 4.0, and how these are likely to impact the industry’s evolution.
Through a combination of sessions led by experts from the likes of GSK and Pall Biotech, as well as group discussions with your peers, you will learn how to bring a biopharmaceutical drug to market. Each day will be chaired by a different member of the BioProcess International Academy faculty and there will also be time for networking and Q&As with the experts.
Learn More