Voices of Biotech
Podcast: MilliporeSigma says education vital to creating unbreakable chain for sustainability
MilliporeSigma discusses the importance of people, education, and the benefits of embracing discomfort to bolster sustainability efforts.
- Sponsored Content
BPI Contributor
December 3, 2021
20 Min View
Date: Dec 3, 2021
Duration: 20 Min
Sponsored by PerkinElmer
This webcast features: Dr. Adil Mohammad, Staff Fellow, US FDA, Dr. Chikkathur Madhavarao, Biologist, US FDA, Robert Thomas, CSci, CChem, FRSC, Principal Consultant, Scientific Solutions
Biotechnology products (biologics) are often produced from mammalian cells grown in large-scale bioreactors. The dynamic environment within the bioreactor is comprised of growth media along with cells, proteins, nutrients of organic and inorganic origin, metabolic waste products and metal ions. Metal ions can act as enzyme cofactors and can directly influence the kinetics of biochemical reactions that may also influence the biological production and quality attributes of therapeutic proteins. As a result, variations of the metal content in the cell culture media can affect the cell growth and production of the biologic product as well as its critical quality attributes (CQAs). The webcast will focus on the use of inductively coupled plasma mass spectrometry (ICP-MS) to study the role of metals in the production of biologics.
The first two speakers will describe an ICP-MS analytical method to determine the composition of a panel of metals in different cell culture matrices, which was then used to validate and screen cell culture media, determine changes in metal concentration during cell growth, evaluate the effect of spikes in metal concentration, and study the impact of zinc supplementation on purity and yield.
The second speaker will focus on the benefits of a novel multi-quadrupole ICP-MS system to mitigate common polyatomic spectral interferences found in cell culture media samples. By utilizing a true quadrupole with dynamic bandpass tuning (DBT) in the collision/reaction cell, it allows the use of any reactive gas to either move the interference away from the analyte mass using MS/MS mode or shift the analyte mass from the interference using Mass Shift mode. The mechanism of interference removal will be demonstrated using examples of metals commonly monitored in cell culture media.
Three takeaways from this webcast:
Complexity of cell culture media with large variations in metal concentrations.
Trace-element analysis of cell culture media poses challenges for conventional ICP-MS.
Multi-quadrupole ICP-MS offers unique interference removal capabilities, improving accuracy and lowering detection limits.
This webcast is no longer available.
About the Author(s)
You May Also Like
Upcoming Webcasts
Jun 17 - Jun 20, 2024
Jun 17 - Jun 20, 2024
Our Biopharmaceutical School is the perfect course to gain a well-rounded understanding of the Biopharmaceutical industry. Over three days, you will understand the principle steps in the biopharmaceutical lifecycle, including: raw material selection, up and downstream processes and core technology. Additionally, you will be familiarised with the latest industry trends, including Industry 4.0, and how these are likely to impact the industry’s evolution.
Through a combination of sessions led by experts from the likes of GSK and Pall Biotech, as well as group discussions with your peers, you will learn how to bring a biopharmaceutical drug to market. Each day will be chaired by a different member of the BioProcess International Academy faculty and there will also be time for networking and Q&As with the experts.
Learn More