Hydrolysates are complex media supplements composed of many as well as different types of components. Predicting cell culture performance of a particular lot remains an elusive target, other than by small scale cell culture testing which consumes valuable time, effort and resources. In order to reduce the need for incoming acceptance tests by our customers, FrieslandCampina Domo plans to develop a simple analytical assay that accurately predicts cell culture performance. Our Quality by Design project has provided information at the individual component level and cell culture performance data of many lots of Proyield soy SE50MAF-UF. FrieslandCampina Domo is currently using these results in a statistical approach to develop the predictive model. This will form the basis for a new and validated outgoing QC test. Working in close collaboration with many of our customers we aim to match the hydrolysate to their specific needs.