AAV Empty/Full Ratio Assessment Using the Octet® AAVX Biosensors

Consider a novel, rapid, and high-throughput method for determining AAV E/F ratio using Octet^® Biolayer Interferometry (BLI) in conjunction with Octet^® AAVX Biosensors. This innovative technique analyzes intact viral capsids and is ideal for screening both crude and purified samples, facilitating a faster more efficient workflow.

By leverages the high precision and consistency of AAVX biosensors, the assay is ideal for screening in the upstream and downstream bioprocessing stages of AAV development and manufacturing. The method involves the saturation of the biosensor surface with AAV particles, effectively normalizing capsid titer between samples. Consequently, a single measurement suffices to determine the E/F ratio, thereby eliminating compounded errors inherent in other methods.