Eshmuno™ Resins for Biochromatography

Eshmuno™ is a new and unique family of ion-exchange resins that are designed specifically for highly productive downstream bioprocessing. The first member of the family, Eshmuno™ S, is a smart cation exchanger designed for fast and efficient purification of antibodies in direct capture and steps following protein A chromatography. Eshmuno™ Q is the newest family member. This smart anion-exchange resin coupled Merck KGaA’s renowned tentacle structure with the new hydrophilic polyvinyl ether base matrix. As a result, Eshmuno™ Q offers outstanding results in typical anion-exchange applications, such as removing impurities in flow-through mode or separating blood factors in plasma processing.

Superior Productivity for Biomolecule Purification

Eshmuno™ Q exhibits a superior binding capacity for various biomolecules. Figure 1 shows the dynamic binding capacity (DBC) of Eshmuno™ Q for selected macromolecules at different flow rates. High flow rates (2 minutes residence time correspond to ~600 cm/hour) do not affect the high binding capacities at low flow rates.

Figure 1: ()

More Stability

Unlike conventional anion-exchange resins, Eshmuno™ Q is intrinsically stable against alkaline solutions used in column sanitization. Figure 2 shows the increased stability of Eshmuno™ Q during storage in 1 M sodium hydroxide solution. Eshmuno™ Q retains its high binding capacity, whereas other anion-exchange resins suffer considerable reductions.

Figure 2: ()

Tangible Savings in Cost and Development Time

Eshmuno™ Q showed remarkably sharp elution of the target molecule in a wide range of applications. Figure 3 shows the elution profile of purified MAb03 at pH 9.5 with 2 minutes residence time (CV = 1 mL), compared with the profile of another surface-grafted anion exchanger. Eshmuno™ Q elutes the antibody in a sharp peak of 12.4 mL; the other resin’s eluate volume is 27.6 mL.

Figure 3: ()

The 130% higher concentration of the target molecule and the 60% reduced elution volume render further concentration steps unnecessary, saving money on buffer solutions and consumables. Our Global Applied Technology Department supports Eshmuno™ users with a global network of applications centers on three continents and with renowned training programs.

About the Author

Author Details
Jessica Schrampf is marketing manager for LSS, 480 South Democrat Rd, Gibbstown NJ 08027; 1-856-423-6300; [email protected]; www.emdchemicals.com.