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Leveraging dPCR Techniques to Quantitate Lentivirus Particles

BPI Contributor

September 29, 2023

20 Min View
Leveraging dPCR Techniques to Quantitate Lentivirus Particles

Date: Sep 29, 2023

Duration: 20 Min

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This webcast features: Samyuktha Shankar, Scientist II, Field Applications, BioProduction Group, Thermo Fisher Scientific.

Digital PCR (dPCR) technology is being increasingly adopted in research, diagnostics, bioproduction, and environmental testing for absolute quantitation of nucleic acids.

In this webinar, Thermo Fisher Scientific will discuss leveraging dPCR for absolute quantitation for lentiviral samples. They’ll demonstrate how this technique reduces variability, and improves accuracy and analytical sensitivity, even in high-background conditions.

Key Takeaways:

  • Leveraging LTR-based quantitation to enable correlation of total (genomic) and infectious viral particles.

  • Measuring lentiviral vector titer and provirus copy number in transduced cells by digital PCR.

  • Understanding the importance of lentiviral characterization to comply with regulatory guidance.

  • Using the combination of commercially-available kits, instrumentation, and software for sensitive and accurate data without sacrificing time and resources.

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