Scaling Up a MAb Purification Process Using Continuous Chromatography

In batch-mode chromatography, operating conditions do not allow full use of a media’s total capacity, leading to a subutilization of the media (1, 2). Sequential multicolumn chromatography (SMCC) is an open-loop process for the separation of multicomponent mixture buffers (3, 4). The concept behind SMCC is to perform different steps in smaller columns (from two to eight columns, compared with batch chromatography) connected in series, to maximize media use without product loss. BioSC® Lab is an automated system based on the SMCC process, designed and provided by Novasep.

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