Accurate and Rapid IgG Titer Determination for Clone Selection and Bioprocess Development

Figure 1.

Accurate and rapid IgG titer determination is critical in the selection of cell lines and culture development for antibody production. ForteBio’s Octet QK and Octet RED Systems provide rapid and accurate IgG quantitation in a label-free format that is easy to use. The Octet family of instruments offers analytical capabilities that provide greater value in applications where existing methods such as HPLC and ELISA have limitations in throughput, performance, workflow, and ease of use.

Principle

Different protein concentrations result in different binding curves, which are plotted against their known concentrations to generate a standard curve. The concentration of unknown samples is calculated based on the binding rate from the standard curve.

Key Features of the Octet QK and Octet RED for Antibody quantitation

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Key Bioprocess Applications