Voices of Biotech
Podcast: MilliporeSigma says education vital to creating unbreakable chain for sustainability
MilliporeSigma discusses the importance of people, education, and the benefits of embracing discomfort to bolster sustainability efforts.
August 10, 2017
Sponsored by Tosoh Bioscience
Single-chain variable fragments (scFv) are antibodyderived molecules with several advantages over full-length IgGs, including rapid target access and good tissue penetration. Straightforward and efficient capturing solutions similar to protein A for monoclonal antibodies (mAbs) may pave the way for the future success of this class of molecules. Herein we describe general conditions for capturing scFv from Escherichia coli with TOYOPEARL® AF-rProtein L-650F and subsequent optimization to achieve efficient purification.
General Guidelines for CapturinG The κ light chains of mAbs, Fabs, and scFv bind to protein L at neutral pH and physiological conductivity, whereas host-cell proteins (HCPs) flow through. The bound product can then be recovered at pH 2.0–3.5. Generic conditions developed for TOYOPEARL AF-rProtein L-650F are described in Table 1.
Login to read the full PDF of the application note.
You May Also Like