Voices of Biotech
Podcast: MilliporeSigma says education vital to creating unbreakable chain for sustainability
MilliporeSigma discusses the importance of people, education, and the benefits of embracing discomfort to bolster sustainability efforts.
Sponsored by Bio-Rad
IgM antibodies are one of the fastest growing groups of diagnostic and therapeutic candidates. Unlike IgGs, IgMs cannot be purified by affinity chromatography due to their physiochemical properties. The large size of IgMs presents an additional purification challenge. Bio-Rad has developed a new strong anion exchange (AEX) resin — Nuvia HP-Q — to overcome the multiple issues faced when purifying large biomolecules. We show the purification of an acidic IgM antibody with capture purification using Nuvia HP-Q and polish purification using mixed-mode CHT Ceramic Hydroxyapatite Media. In initial experiments, the capture step removed process-related impurities while polishing with ceramic hydroxyapatite depleted these impurities further. The described purification strategy is simple and scalable. The studies resulted in product purity >99% IgM, as measured by size exclusion chromatography and SDS-PAGE analysis. This purity makes the IgM suitable for diagnostic applications.
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