October 2019 Supplement

Monoclonal antibody (MAb) expression systems typically use signal peptides to ensure secretion of antibodies into cell culture media. Although that reduces the complexity of purification and prevents the need for cell disruption, it does require using expensive and time-consuming techniques to separate cells from antibody-containing cell culture fluids. In this study, we describe our tests of the novel Sartoclear Dynamics Lab V system (Sartorius S Lab Instruments GmbH and Co. KG) for rapid clarification of cell culture media without requiring centrifugation or additional costly equipment. The filtration system was tested and compared with the method currently used to separate cells from antibody-containing cell culture fluids. That standard clarification method includes an initial centrifugation step followed by filtration of the cleared supernatant through a 0.45-μm filter on a vacuum-driven bottletop filter. Further Reading Kempken R, Preissmann A, Berthold W. Clarification of Animal Cell Cultures on a ...