Beyond Standard Protein A Resin: Establishment of an Improved DSP Affinity Platform Step for Monoclonal Antibodies

This webcast features: Manuel Matos, PhD, Field Application Scientist, Thermo Fisher Scientific and Marco Baldazzi, DSP Research Laboratory Technician, Menarini Biotech.

While protein A affinity chromatography is the most commonly used method for monoclonal antibody (MAb) capture in clarified harvest coming from CHO cell cultivation, one significant challenge is the high cost of the media required when using these resins. Therefore, it’s crucial to evaluate alternative strategies that may reduce costs and increase productivity without affecting product quality attributes.

In this study, MabCapture^TM C affinity resin has been compared with a Protein A resin used in a DSP platform, showing a higher dynamic binding capacity while keeping the product’s critical quality attributes within acceptable limits. Furthermore, the adsorbent’s robustness and reusability when scaling up makes MabCapture C affinity resin the ideal choice for bioprocessing of MAbs.

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