Corning X-WASH System: DMSO Reduction of Cryopreserved Human Mesenchymal Stem Cells

X-Wash reduction of DMSO

Figure 1: Human mesenchymal stem cell (hMSC) viability using an X-WASH system

Cryopreservation is a necessary part of workflows for both autologous and allogeneic therapies. The ability to cryopreserve cells for cell therapy increases the range of administration, shelf life, and time for safety testing. Cryoprotectants such as dimethyl sulphoxide (DMSO) often are added to freezing media to increase post-thaw cell survival. However, DMSO itself can be cytotoxic, so reducing its final concentration can be necessary. Below, I demonstrate how the Corning X-WASH system can reduce the amount of DMSO used in cryopreserved cells through a semiautomated, closed system. Using the Corning X-WASH system, we reduced DMSO concentration significantly for cryopreserved bone marrow-derived human mesenchymal stem cells (hMSC) while maintaining high cell recovery and viability.

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