Innovation in Tangential Flow Filtration: Holder-Less Self-Contained Flat Sheet Cassette Device

This webcast features: Mark A. Perreault, Director, Research and Development, Repligen

Tangential Flow Filtration (TFF) has been a cornerstone technology in bioprocessing, enabling the separation and concentration of biomolecules with precision and efficiency. In this study, we introduce a groundbreaking innovation in TFF technology with the launch of the first holder-less self-contained true flat sheet cassette device. This device represents a paradigm shift in TFF, eliminating the need for traditional cassettes holders while delivering performance comparable to conventional formats. In this webinar, Repligen presents the TangenX SC Device, discuss benefits of a holder-less TFF process, and demonstrate comparability to traditional TFF cassettes while processing a crude monoclonal antibody (MAb) feed.

The introduction of the holder-less self-contained true flat sheet TFF device represents a significant advancement in TFF technology. Its versatility across bioprocessing applications and 80% reduction in process set up time opens new possibilities for simplifying and optimizing biomolecule manufacturing processes while maintaining product quality and yield.

Key Takeaways:

  • The novel TFF device introduces a holder-less design, simplifying the setup and reducing the risk of cassette installation issues, thereby improving process efficiency and reliability.
  • Easily scale from other cassette formats because the same channel path length is maintained across cassette formats with the same pressure drop profile from feed to retentate.
  • The device incorporates a true flat sheet cassette design, ensuring uniform flow distribution across the membrane surface, resulting in consistent performance and enhanced product recovery.
  • Testing reveals that the holder-less device delivers comparable performance to traditional cassette formats, achieving equivalent separation efficiency, flux rates, and fouling resistance.
  • To validate the device’s capabilities, we demonstrate its performance using both bovine IgG and a crude mAb feed stream. The device consistently achieves comparable recovery while effectively concentrating and diafiltering, demonstrating its suitability for critical bioprocessing applications.