MAb Aggregate Removal Through Ion-Exchange Chromatography: In Bind/Elute and Flow-Through Mode with TOYOPEARL NH2-750F

A polishing step for the purification of a monoclonal antibody (MAb) using TOYOPEARL NH2-750F was developed. In general, anion-exchange resins can be used in bind and elute (B/E) mode as well as in flow- through (FT) mode. Both options were evaluated. To increase the amount of aggregates of the test sample, a MAb was aggregated by acidic incubation and subsequently diluted to 1 g/L in loading buffer.

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