December 18, 2020
Understanding the functionalities of chromatography resins can improve the product yield and purity in a biotherapeutic purification workflow. Ion-exchange (IEX) chromatography separates biomolecules on the basis of charge. For several reasons, it is the most widely used separation tool for purification of biopharmaceutical products. IEX is a well-characterized purification method with high binding capacity and flexible selectivity. It also works with mild operating conditions that help to preserve the biological activity of a biopharmaceutical drug substance. That versatility enables several options for capture, intermediate, and polishing steps in downstream processing.
In this eBook, BPI’s senior technical editor, Cheryl Scott, explores practical considerations for IEX chromatography workflows. The author overviews critical criteria for resin selection, including particle size, flow rate, and dynamic binding capacity. Then Scott describes specific applications in which IEX can be an ideal bioseparation tool, including nonaffinity capture of proteins and antibodies, high-resolution separation of charge variants developed during antibody production, purification of viruses and other large molecules, and removal of process-related impurities.
Fill out the form below to read the eBook and learn more about IEX chromatography now.
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