Sartobind® pico and Sartobind® nano on Akta® – Optimizing Conditions for Liquid Chromatography Systems

Sartobind pico and Sartobind nano are the laboratory-scale mem-bers of the Sartobind membrane adsorber family. Accordingly, their small size and high throughput make them perfect for use on automated chromatography systems such as the popular Akta series offered by GE Healthcare Life Sciences.While both the pico and nano capsules are designed for high flow rates, the array of valves, pumps, monitors and the flow restrictor found in a typical flow path for gel chromatography can cause unacceptable pressures at the designed flow rates of the capsules. Although a lower flow rate will reduce the pressure in the system, this does not take advantage of the rapid processing offered by Sartobind membrane adsorbers. A method designed to maintain ideal conditions without generat-ing excessive pressure is described along with an alternative Flow Path confi guration for a typical Akta system.