Efficiency Increase for Single-Use Cell Removal (Midstream) Using Filter Aid

Continuous process optimization is a key factor in the biotech in-dustry. With higher and higher particle loads (>108 cells/ml), standard technologies for cell removal (midstream processing) – e.g. centrifugation, separation, membrane- and depth filtration – find their limits. The goal of the following study was to focus on making the midstream process more economical and investigate the efficiency increase for the cleaning of fermentation broths through alluvial filtration (see box) and its ease of scalability. This technology leads to a maxi-mum product yield and highest economic efficiency.