Biopharmaceutical Production in Fed-Batch CHO Cell Culture

Chinese hamster ovary (CHO) cells are used widely in the biopharmaceutical industry for the production of recombinant proteins. In times of rapid growth in the biopharmaceutical market with monoclonal antibodies (MAbs) and biosimilars, the use of chemically defined (CD) feeds and culture media for CHO mammalian cell culture is crucial. Currently, strict regulatory norms for the biopharmaceutical industry has led to the wide use of animal-component–free (ACF) culture processes, including CD media and feeds. A business requires significant amounts of time and investment to develop an optimal CD fed-batch medium solution that fits the needs of both the company and its process. Many companies might not have the resources to develop their own CD media.

Kerry now offers two CD media for extended growth and enhanced recombinant protein production for CHO suspension cultures. AmpliCHO CD medium is a protein-free, serum-free medium that has been optimized so that it can be seamlessly combined with Kerry’s individual plant-based hydrolysates and with Kerry’s series of Sheff CHO systems. MAM-PF®77 medium (manufactured by BioConcept, distributed by Kerry) is a richer CD protein-free, serum-free medium that is also compatible with Kerry’s complex supplements. Kerry also has developed a new CHO cell-line–specific CD and ACF feed. AmpliCHO CD feed works synergistically with Kerry’s CD media to improve viable cell density, cell viability, and recombinant protein production. This feed is available with or without added glucose.

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