The widespread use of multicolor immunophenotyping to help identify and quantify specific lymphocyte subsets in patient specimens represents a major advance in healthcare. Consistent quality control and the careful standardization and validation of assays, instruments and methodologies are now essential needs of a flow cytometrist.
In this application note, we have demonstrated the ease of use, reproducibility of automated setup and compensation, and resolution sensitivity of the BD FACSLyric™ Flow Cytometer platform. These features may be useful for generating consistent results for longitudinal or multisite studies in which the same assay is used to acquire data on different days, on different instruments or by many operators with varying levels of expertise.