Production of Transient Lentiviral Vectors in HEK 293T Cells: Cultivation on Fibra-Cel Disks in a Single-Use, Packed-Bed, Stirred-Tank Bioreactor

Figure 1: Functional titers (TU/mL) of the unconcentrated LV supernatant determined by using flow cytometric detection of GFP-positive cells

Although demand for lentiviral vectors (LVs) for cell and gene therapy is increasing, the standard two-dimensional culture systems used to produce LVs present significant disadvantages. Current bottlenecks in LV production are caused mainly by such disadvantages. Switching to use of bioreactors can eliminate those problems because bioreactors offer the benefits of process automation, tight regulation of production conditions, and reduced labor input. The study reported herein was carried out by the group of David Parsons at the University of Adelaide. It was one of the first attempts at transient LV production in a stirred-tank bioreactor with cells adherently grown on Fibra-Cel disks.

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David Solbach is scientific communications manager of bioprocess at Eppendorf AG, Rudolf Schulten-Str. 5, 52428 Jülich, Germany, 49-2461-980-168; [email protected].