Push-Button Simplicity: Automatic Fermentation with the BioFlo® 120 Auto-Culture Mode

Learning to use a bioprocess controller is a complex endeavor. Even if someone has previous bioprocess experience, moving to a new software platform can entail much learning and reduce process efficiency. With the auto-culture modes of the Eppendorf BioFlo 120 bioprocess control station, a user can select with a push of a button either a predefined Escherichia coli batch fermentation protocol or a Chinese hamster ovary (CHO) batch cell culture process.

Proof of Concept: E. coli Auto-Culture Fermentation
The auto-culture modes are populated with setpoints tested by the Eppendorf applications development team. A user needs only to select the vessel size and type and make standard preparations (e.g., sensor and pump calibration, vessel preparation). Once ready, the user simply presses the “play” button. To demonstrate the ease of use of the auto-culture mode, we performed an E. coli batch fermentation using a strain that produces green fluorescent protein (GFP). The setpoints for each control loop were auto-populated as outlined in Table 1.

Login to read the full PDF of the application note.