Strategies to Address Large Molecule Purification Challenges: Case Studies on Plasma Proteins and Virus Purification

This webcast features: Artur Stanczak, Field Application Specialist, Process Chromatography, Bio-Rad Laboratories.

Large molecule purification presents significant challenges due to their considerable size. For instance, Immunoglobulin M (IgM) molecules, ranging from 900 to 1,000 kD, play pivotal roles in mammalian immune responses and find extensive applications in diagnostics and therapeutics. Purification of such molecules is particularly intricate owing to several factors including their weak Protein A binding sites, limited dynamic binding capacity, and slow diffusion rates.

Moreover, IgMs exert notable effects on chromatographic purification processes. Their inability to penetrate small pores necessitates higher pore volume utilization, resulting in sluggish diffusion and mass transfer kinetics within traditional chromatography resins. Consequently, this diminishes binding capacity and resolution, thereby complicating purification efforts.

This webinar will delve into innovative strategies to surmount these challenges, employing design of experiments (DOE) methodologies and offering insightful case studies centered around plasma protein and virus purification. These real-world applications showcase tangible enhancements in productivity and overall process economics within large molecule purification.

 Key Takeaways:

Just fill out the form below to watch the recorded webcast now.