Assay Miniaturization Saves Time, Labor, and Resources to Boost Overall Efficiency and Productivity

ELISA-based sandwich assays have been a traditional workhorse across the drug discovery continuum for many years, but they have significant limitations. Gyros® offers a more efficient solution for performing high-throughput, automated immunoassays on a miniaturized, fluorescence-based platform.

A head-to-head comparison of the microfluidics-based Gyrolab® technology and ELISA demonstrates clear distinctions:

What Is Gyrolab?

Gyrolab is a bioanalytical, automated, fluorescence-based system that employs microfluidics technology to perform immunoassays at nanoliter scale. At the core of the Gyrolab system is a compact disc (CD) microlaboratory, in which are embedded microstructures containing affinity capture columns. By integrating microfluidic CD technology with sensitive detection methods and intuitive software, this automated system yields quantitative assay data and highly reproducible results.

How Does It Work?

Designed based on microfluidic flow-through principles, Gyrolab’s CD-based microlaboratory allows only brief contact between the immobilized solid phase and the sample. This permits the desired reactions to take place while preventing undesirable nonspecific binding and matrix effects that can compromise the performance of static assay platforms, such as microplate-based ELISAs. The combination of affinity columns with a high capacity for the biotinylated capture reagent and fluorescently labeled reagents contribute to the broad dynamic range of Gyrolab. The miniaturized assay format uses nanoliter volumes, minimizing the consumption of precious samples and reagents. Extremely small sample volumes combined with short assay times makes multiplexing superfluous; Gyrolab combines the robustness of singleplexing with the flexibility of multiplexing.

Why Downsize Your Assays?

Value-Added Applications

Assay miniaturization and automation enable high-throughput screening and analysis of drug compounds, enhancing productivity across drug discovery and preclinical testing. As the numbers of biopharmaceuticals and especially protein therapeutics continue to increase in corporate pipelines, demand is growing for rapid, robust, efficient, and reproducible tools and methods for evaluating a compound’s safety, efficacy, metabolic, and toxic properties. Gyrolab offers particular advantages for applications such as pharmacodynamics (PD), pharmacokinetics (PK), antidrug antibody assays, detection of bioprocess impurities, biomarker assays. For protein production scale-up and optimization of bioprocesses, Gyrolab’s miniaturized format, fast turn-around time, broad dynamic range, and reproducibility provide tangible benefits.