This webcast features: Samyuktha Shankar, Scientist II, Field Applications, BioProduction Group, Thermo Fisher Scientific. Digital PCR (dPCR) technology is being increasingly adopted in research, diagnostics, bioproduction, and environmental testing for absolute quantitation of nucleic acids. In this webinar, Thermo Fisher Scientific will discuss leveraging dPCR for absolute quantitation for lentiviral samples. They’ll demonstrate how this technique reduces variability, and improves accuracy and analytical sensitivity, even in high-background conditions. Key Takeaways: Leveraging LTR-based quantitation to enable correlation of total (genomic) and…
Webinars
Pre-Packed Chromatography Columns for Risk Reduction: Statistical Process Control Analysis Demonstrates Performance Consistency
This webcast features: Adam Nelson, Chromatography Product Manager, Repligen. The OPUS® manufacturing team packed, tested, and gathered process data for > 12,000 OPUS Columns spanning 250+ resin types. Consistency is observed for efficiency and asymmetry values across each sizing segment within the OPUS Column portfolio based on statistical analysis of frequently produced configurations. Due to the control of OPUS® Pre-packed Chromatography Column input parameters, users benefit from confidence in predictive results for output performance metrics. Key Takeaways: Streamline your chromatography…
Managing Complex Considerations With Pharmaceutical Facility Design
This webcast features: Patrick Nieuwenhuizen, Director, Principal Consultant, Quality Management & Compliance & Gerardo Gomez, PhD, Director, Service Line Lead, QMC, PharmaLex. Pharmaceutical manufacturing facilities, regardless of where they are, must meet GMP principles and procedures. There are, however, many other important and often poorly understood considerations with facility design. Beyond quality and compliance and country-specific expectations, facility design has a huge impact on decisions that both the sponsor and the contract manufacturing organization (CMO) must make. The issues faced…
The Use of Retrovirus-Like Particles (RVLP) to Evaluate Viral Clearance in Downstream Processing
This webcast features: David Cetlin, Senior Director, MockV Products, Cygnus Technologies. Type C Retrovirus-Like Particles (RVLP) are endogenously produced during CHO cell expression. As such, regulatory agencies require proof that downstream process steps can effectively remove or inactivate retrovirus. A model mammalian virus, Xenotropic Murine Leukemia Virus (XMuLV) is typically used to demonstrate RVLP clearance. However, the establishment of RVLP quantification methods has made it possible to track the removal of actual endogenous RVLP throughout the downstream process. Additionally, the…
Unlocking the Potential of CHO Cells: Advancing Bioproduction with Next-Generation Vector Design and Model-Guided Cell Line Development
This webcast features: Scott Estes, Head of Cell Line Development, Asimov. Traditional cell line development to support bioprocessing has typically relied on a “one-size-fits-all” vector, which can streamline development, but in most cases can lead to suboptimal expression levels, particularly for complex multi-specific molecular architectures. The commercial consequences are increased cost of goods, reduced facility flexibility, and in the worst case the inability to support commercial demand. In this webinar, Asimov presents their CHO Edge platform, which builds on the…
Scalable AAV Vector Purification Workflow Using AEX and Mixed-Mode Chromatography
This webcast features: William H. Rushton, MS, Process Chromatography Scientist, Bio-Rad Laboratories. Recombinant adeno‐associated viruses (rAAV) are among the most promising vectors for long‐term gene transduction. These viruses have a high degree of safety, making them ideal for gene therapy applications. Significant progress has been made in improving rAAV vector production and purification. In this study, different chromatography workflow solutions were explored to purify rAAV8. Experimental conditions and data will be presented on using anion exchange (AEX) and…
Accelerate Upstream Process Development With Data-Driven Feeding Strategy Optimization
This webcast features: Chris Schultz, PhD, Team Leader, Centre for Process Innovation, National Biologics Manufacturing Centre. Accelerated timelines and cost reduction are critical in today’s bioprocessing environment, but it is imperative to ensure product quality is unaffected by process modifications. At-line and on-line technologies that inform bioprocess parameters and cell-feeding strategies are needed to develop processes that deliver high-quality products while reducing manufacturing costs. At the Centre for Process Innovation (CPI), bioprocessing experts work with partners across diverse markets in…
Planova™ Webinar – Addressing Continuous Bioprocessing Challenges From Virus Removal Perspective
This webcast features: Kathleen McLaughlin, Senior Scientist, BioProcess R&D, Merck & Hironobu Shirataki, PhD, Senior Consultant, Scientific Affairs, Global Marketing, Bioprocess Division, Asahi Kasei Medical. There are two presentations during this webcast. Evaluation of the Planova™ BioEX Virus Removal Filter in Continuous Bioprocessing of a Monoclonal Antibody by Kathleen McLaughlin Standard batch monoclonal antibody biologics manufacturing processes are costly and time-consuming. A switch to intensified continuous processing enables much higher productivity, notably > 1.5 g/L/day over 25+ days of bioreactor…
Highly Efficient Capture of Low Titer Fusion Molecule With Novel Protein A Membrane
This webcast features: Florian Knoll, Scientist, Bibitec GmbH. Over the last decades, innovations have led to rapid development and growth in biopharmaceutical production, which has gone hand in hand with the urgent need for new medicines and treatments for today’s increasingly fast-growing society. In particular, upstream processing has benefited from new technologies in recent years, enabling high titers of therapeutic antibodies and antibody related products in the order of several kilograms. Therefore, downstream processing must now catch up to ensure…
Development of an IEX Purification Process for Lentiviral Vectors
This webcast features: Sushmita Koley, PhD, Senior Scientist, Process Chromatography, Bio-Rad Laboratories. In recent years, gene therapy treatments have increased significantly requiring more efficient processes to purify and deliver these therapeutics. Lentiviral vectors (LVV) are a type of retrovirus and are an effective tool for gene delivery. In this webinar, data will be presented on a high-throughput screening strategy that was employed for LVV purification which provided high yield as well as acceptable HCP clearance. An initial high-throughput screening experiment…