Upstream

Unmatched Productivity and Seamless Scalability: Advantages of the XCell® ATF System for Upstream Process Intensification

This webcast features: Muhammad Shamim, Field Applications Scientist, Upstream, Repligen. With the constant pressure of meeting aggressive process development timelines while continuously improving upstream cell culture yield and productivity, developing the optimal processes from the beginning is crucial. With over 500 adoptions at process and commercial scale across biopharma globally, the XCell® ATF System is a proven technology for intensified cell culture that exponentially boosts productivity, lowers costs, and expands capacity of existing manufacturing facilities in upstream bioprocessing. In this…

Development of Advanced Feed Media and Supplements for a Robust, Cost-Effective, and High-Productivity Process

This webcast features: Yaron Silberberg, PhD, Chief Scientist, Ajinomoto CELLiST Korea Co., Inc. Cell culture media composition and upstream bioprocess conditions are crucial factors affecting protein yield in the production of biologics and biosimilars. Recently, advanced new techniques were developed that use IT approaches, including computer simulation of bioprocesses, AI tools, and ‘multiomics’ methods for analysis of cellular metabolic pathways. These techniques allow optimization of both process and media composition for maximizing culture performance and protein production. In this webcast,…

A Breakthrough AAV Enhancer to Improve Titers and Reduce Production Costs with Any Serotype, Suspension HEK Cell Type, and Transfection Reagent

This webcast features: Leisha Kopp, Senior Field Applications Scientist, Mirus Bio. With the exponential increase in clinical trials examining recombinant adeno-associated virus (AAV) for gene therapies, significant upstream manufacturing improvements are needed. In this webinar, learn strategies to increase viral yields and reduce production costs by incorporating lipid polymer nanocomplexes (LPNCs) and novel enhancers into suspension HEK-based transient transfection platforms. Review key parameters considered during enhancer development as well as techniques to ensure scalability and seamless adoption from bench to…

Accelerate Upstream Process Development With Data-Driven Feeding Strategy Optimization

This webcast features: Chris Schultz, PhD, Team Leader, Centre for Process Innovation, National Biologics Manufacturing Centre. Accelerated timelines and cost reduction are critical in today’s bioprocessing environment, but it is imperative to ensure product quality is unaffected by process modifications. At-line and on-line technologies that inform bioprocess parameters and cell-feeding strategies are needed to develop processes that deliver high-quality products while reducing manufacturing costs. At the Centre for Process Innovation (CPI), bioprocessing experts work with partners across diverse markets in…

Enabling Faster Feed Strategy Optimization with Rapid Spent Media Analysis and Data Visualization Tools

This webcast features: Milla Neffling, PhD, Bioprocessing Market Segment Manager, 908 Devices. Optimization of feed strategy to boost process productivity and meet critical quality attributes can greatly benefit from using real-time analytics to gain key insights into the process. Frequent measurements of key nutrients and metabolites in spent media also come with the challenge of generating daunting amounts of data. Bioprocess data visualization can therefore be a cumbersome and lengthy task when multiple dynamic key factors are considered. We highlight…

Benefits of Real-Time Analytics to Overcome Common Cell Culture Challenges

This webcast features: Nick Randall, Bioprocessing Product Manager, 908 Devices. Faster development cycles, intensified processes, and automation of process control are key initiatives in biotherapeutics manufacturing. However, offline analytics requiring manual intervention are commonly used to monitor critical and time-sensitive process parameters. We describe a new automated approach for optimal growth and production, leveraging sensitive, on-line (sample-free) monitoring of glucose and lactate with automated feed control, and its impact on growth/viability, lower toxic metabolite, and improved PQA. Key takeaways: Explore…

Benefits of Hollow Fiber Filter Cell Expansion

This webcast features: Stuart Gibb, PhD, Scientific Strategy Lead, Terumo Blood and Cell Technologies. Cell therapy requires cells to be healthy and active throughout the entire manufacturing process for optimal endpoint efficacy. A key part of this process is cell expansion. The Quantum Flex hollow-fiber filter technology provides an effective cell culture environment to provide ready access to fresh media, waste removal, and gas exchange over micrometer distances and works with suspension and adherent cell culture. In this environment, we…

Analytical Tools to Improve Production of Full Capsids in Initial Stages of AAV Process Development

This webcast features: Ivana Petrović Koshmak, PhD, Head of Upstream Process Development, BIA Separations, now a Sartorius company. Empty AAV capsids are known contributors of immunogenicity in gene therapy products. Reducing them below 10% during downstream process is difficult if they constitute the vast majority of total AAV produced in harvest – the solution is a further optimization of harvest. Determining the factors that improve production of full AAVs is possible at the initial stages of upstream process development, with…

Drug Discovery: Screening Approaches for Rapid Assessment of Target Tractability

This webcast features: Nuska Tschammer, Head of DEL Lab Operations, WuXi AppTec. In recent years, increasing use of genetic, transcriptional and knock-out technologies led to numerous biologically validated targets, many of them in the category “first-in-class”. The core purpose of experimentally based tractability assessments is to evaluate if the target of interest can be modulated by a chemical entity. In the past, extensive HTS screening efforts were initiated to find starting points for the small molecule drug discovery. Today, direct…

Improve Your Cell Line Development Workflows with Analytical Tools

This webcast features: Dirk Müller, PhD, Manager of Media & Process Development, Sartorius, Lukas Klein, Scientist, Sartorius. Cell line development involves screening thousands of clones to find those that are stable, produce high yields of the bioproduct, and exhibit desired critical quality attributes (CQAs). While there are multiple CQAs that need to be monitored early in the development process, product yield, cell count and viability assessment remain the main attributes by which process conditions are optimized. Incorporating analytical techniques into…