July 1, 2008
Figure 1.
Accurate and rapid IgG titer determination is critical in the selection of cell lines and culture development for antibody production. ForteBio’s Octet QK and Octet RED Systems provide rapid and accurate IgG quantitation in a label-free format that is easy to use. The Octet family of instruments offers analytical capabilities that provide greater value in applications where existing methods such as HPLC and ELISA have limitations in throughput, performance, workflow, and ease of use.
Principle
Different protein concentrations result in different binding curves, which are plotted against their known concentrations to generate a standard curve. The concentration of unknown samples is calculated based on the binding rate from the standard curve.
Key Features of the Octet QK and Octet RED for Antibody quantitation
Real-Time and Label-Free Detection
Correlates Well to HPLC
Simple Assay Set-Up: Assay crude cell lysate samples without centrifugation or serial dilutions
Direct Readings from Crude Sample Media: Octet biosensors detect binding only at the sensor surface with minimal interference from biological sample media or refractive indices. Proteins can be assayed in cell culture media or crude lysates.
Rapid Results: Octet runs up to eight samples in parallel and up to 96 samples in unattended operation. A full 96-well plate can be analyzed in 30 minutes.
Key Bioprocess Applications
Clone selection
Early cell culture screening
Cell line optimization and development
Manufacturing
Protein Purification
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