November 5, 2024
Sponsored by Sartorius
Consider a novel, rapid, and high-throughput method for determining AAV E/F ratio using Octet® Biolayer Interferometry (BLI) in conjunction with Octet® AAVX Biosensors. This innovative technique analyzes intact viral capsids and is ideal for screening both crude and purified samples, facilitating a faster more efficient workflow.
By leverages the high precision and consistency of AAVX biosensors, the assay is ideal for screening in the upstream and downstream bioprocessing stages of AAV development and manufacturing. The method involves the saturation of the biosensor surface with AAV particles, effectively normalizing capsid titer between samples. Consequently, a single measurement suffices to determine the E/F ratio, thereby eliminating compounded errors inherent in other methods.
Suitable for analysis of crude and purified AAV samples
No sample purification or enzymatic digestion required (analyzes intact viral capsids)
Compatible with multiple AAV serotypes
Efficient: complete assay in as little as 30 minutes
Cost-effective: AAVX biosensors can be regenerated and re-used multiple times
Leverages the high precision and consistency of Octet® AAVX Biosensors
Ideal for screening applications in upstream and downstream process development
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