Address Challenges of Bispecifics and Antibody Fragments with Novel Affinity Resins

Advancements in the field of therapeutic antibody design and upstream production continue to challenge traditional downstream purification processes.  As the complexity and titer of target molecules increase, both recovery and separation from the various product related impurities becomes non-standard, and exploration of advanced chromatography tools is essential for efficient purification schemes.

One promising technique is the use of affinity resins that target different antibody subdomains distinct from protein A to increase yield and purity of challenging feeds, reducing the burden on subsequent polish steps.

This article presents use-cases from literature where affinity chromatography resins targeting the CH1 region, Fc region, and kappa and lambda light chains are successfully employed to increase purity and yield of various bispecific antibody (bsAb) feeds with challenging impurity profiles.

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