GatorTM AAVX Probes for Rapid and Label-free Quantitation of AAV Serotypes

Adeno associated virus (AAV) capsids are a leading modality for in vivo gene delivery. Complete and precise characterization of capsid particles, including capsid and vector genome concentration is necessary to safely and efficaciously dose patients. In virus development and production, it is important to determine the virus concentration at different stages of the process, optimize the clone used and obtain best production yields. Easy, rapid and cost effective quantitation methods are critical to achieve above goals.

The Gator AAVX probes are a high specificity antibody-based BLI biosensors that enable direct capture and quantitation of different serotypes of AAV in crude lysates, column eluates, cell lysates and cell culture supernatants. BLI is a fast, easy to use and cost effective alternative to traditional time-consuming analytical methods, such as qPCR, ddPCR, Dot blot and ELISA. The Gator AAVX probe uses proven CaptureSelect™ (Thermo Fisher Scientific) high affinity and high specificity anti-AAVX antibody. The probe quantitates many AAV serotypes in minutes using GatorPrme and GatorPlus BLI systems.