Gibco TrypLE Select Enzyme: An Alternative to Animal Trypsin for Cell Dissociation

Ideal for both serum-supplemented and serum-free conditions, TrypLE enzymes are an answer to animal origin-free trypsin-like enzymes. Exhibiting a similar pH activity profile to trypsin, this enzyme cleaves at arginine and lysine. With the TrypLE enzyme’s animal-free origin, hazards from potential pathogenic contaminants have been eliminated; and subject to a controlled fermentation process, TrypLE enzymes are available for supply at any scale.

The TrypLE enzyme is designed to seamlessly fit your workflow. TrypLE enzymes can be substituted for trypsin in existing protocols, is room temperature stable and, in addition, is exceedingly gentle on cells. So gentle, in fact, that the TrypLE enzyme requires no inhibiting agent. Instead, it is inactivated by dilution alone. In addition, TrypLE enzymes boast similar dissociation kinetics to porcine trypsin as well as comparable cell replating, proliferation kinetics, and long-term maintenance.

TrypLE products offer a greener, more economical cell dissociation.

TrypLE Select enzymes are manufactured on dedicated animal origin-free machinery and designed specifically for the bioproduction industry. Select enzymes come in two concentrations, 1X and 10X, and are phenol red-free. Using 10X at full strength can rapidly, but gently, dissociate strongly adherent cells.
Learn more in the datasheet.