Label-Free Monitoring of T-Cell Activation Using Laser Force Cytology™

Chimeric Antigen Receptor T-cell (CAR-T) therapy represents a monumental leap in cancer treatment, harnessing the power of modified T-cells to target and destroy tumor cells. Despite its clinical triumphs, the journey is fraught with challenges such as donor T-cell quality variability, T-cell senescence, and the quest for optimal activation conditions. The FDA underscores the necessity for stringent acceptance criteria for cellular starting materials, given the inherent variability among donors. Effective T-cell activation is pivotal for CAR gene insertion and subsequent cell expansion, yet over-activation poses the risk of Cytokine Release Syndrome (CRS), demanding vigilant monitoring.

The intricate nature of CAR-T biomanufacturing often results in bottlenecks. Inconsistent in-process testing methods underscore the urgent need for robust cellular process analytical technologies (PATs) to ensure product quality and yield optimization. These technologies must be harmonized across R&D, process development, and manufacturing to mitigate costly assay development and comparability issues.

LumaCyte’s Laser Force Cytology™ (LFC™) technology, exemplified by the Radiance® instrument, offers a profound solution. This rapid, label-free method analyzes cellular starting materials and monitors T-cell activation with precision. By leveraging optical pressure and microfluidics, LFC™ provides real-time insights into cellular properties, facilitating the development and monitoring of critical quality attributes (CQAs). This advanced cellular PAT analytic approach enhances the consistency and quality of CAR-T manufacturing, paving the way for more reliable and effective therapies.