Purification of Clinical Grade MAb using Hydrophobic Cation Exchange Chromatography Resin

A three-step non-rProtein A capture process shows how the selectivity afforded by Nuvia cPrime resin can be used to clear a light chain contaminant that has a homologous sequence and similar pI to the target MAb monomer.

The unique balance between the hydrophobic and charged character of Nuvia cPrime allows for a compact process with reduced buffer consumption and feed conditioning.